| Attributs | Valeurs |
|---|
| type
| |
| Is Part Of
| |
| Subject
| |
| Title
| - Dual-Electrode Electrochemical Detection forPoly(dimethylsiloxane)-Fabricated CapillaryElectrophoresis Microchips
|
| has manifestation of work
| |
| related by
| |
| Author
| |
| Abstract
| - The development of a poly(dimethylsiloxane)-based (PDMS-based) microchip electrophoresis system employing dual-electrode electrochemical detection is described. This isthe first report of dual-electrode electrochemical detectionin a microchip format and of electrochemical detectionon chips fabricated from PDMS. The device described inthis paper consists of a top layer of PDMS containing theseparation and injection channels and a bottom glass layeronto which gold detection electrodes have been deposited.The two layers form a tight reversible seal, eliminating theneed for high-temperature bonding, which can be detrimental to electrode stability. The channels can also betemporarily removed for cleaning, significantly extendingthe lifetime of the chip. The performance of the chip wasevaluated using catechol as a test compound. The response was linear from 10 to 500 μM with an LOD (S/N= 3) of 4 μM and a sensitivity of 45.9 pA/μM. Collectionefficiencies for catechol ranged from 28.7 to 25.9% at fieldstrengths between 200 and 400 V/cm. Dual-electrodedetection in the series configuration was shown to beuseful for the selective monitoring of species undergoingchemically reversible redox reactions and for peak identification in the electropherogram of an unresolved mixture.
|
| article type
| |
| is part of this journal
| |
