| Abstract
| - A novel amperometric biosensor for quantification of theelectrochemically inert polar organic solvents based ontyrosinase electrode was preliminarily reported. The biosensor was fabricated by simply syringing an aqueoussolution of tyrosinase/PVAVP (PVAVP: copolymer ofpoly(vinyl alcohol) grafting with 4-vinylpyridine) ontoglassy carbon electrode surface followed by drying themodified electrode at +4 °C in a refrigerator. The currentgenerated from electrochemical reduction of quinone isa probe signal. The biosensor can be used for quantification of polar organic solvents, and its mechanism wascharacterized with in situ steady-state amperometry−quartz crystal microbalance experiments. The detectionlimit, sensitivity, and dynamic range for certain organicsolvents are dependent on the kind and concentration ofthe substrate probe and the hydrophobicity of the immobilization matrix. The response time for all the testedorganic solvents is less than 2 min.
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