| Abstract
| - Nonfluorescent labels were used for an array-formatmultiplex detection of alternative splice junctions ofbreast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detectionscheme using nonfluorescent labels was demonstrated forDNA sequences specific to four BRCA1 alternative splicevariants: Δ(11q) (the last 3309 nt deleted from exon 11),Δ(9, 10) (exon 9 and 10 deleted), Δ(5) (exon 5 deleted),and Δ(5q, 6) (the last 22 nt of exon 5 and the entire exon6 is deleted). This is the first proof-of-concept study toapply SERS-based detection using nonfluorescent labelsto investigate alternative gene splicing. Detection sensitivity of up to 1 fM was demonstrated for the Raman labelschosen to clearly discriminate the splice junctions viaspecific target identification. The proposed approach hasthe potential to become a highly sensitive and selectivetool for comprehensive alternative splicing profiling ofBRCA1 or other genes relevant to specific diseases.
|
