| Abstract
| - In this work, carbon-fiber microelectrode amperometryis used to characterize serotonin exocytosis from murineperitoneal mast cells cocultured with fibroblasts in thepresence of Au nanoparticles. In the case of mast cellexposure to 1 nM 28 nm diameter spherical Au nanoparticles, there is a decrease of greater than 30% in thenumber of successful granule transport and fusion events,greater than 30% increase in the rate of intragranularmatrix expansion, and greater than 20% increase in thenumber of secreted serotonin molecules per granule.These results suggest that nanoparticles interrupt thedense-core biopolymer intragranular matrix and presentthe potential for systematic studies showing how exocytotic function is influenced by nanoparticle size, shape,and composition.
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