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À propos de : Pre-Steady-State Analysis of ATP Hydrolysis by Saccharomyces cerevisiae DNATopoisomerase II. 1. A DNA-Dependent Burst in ATP Hydrolysis        

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  • Pre-Steady-State Analysis of ATP Hydrolysis by Saccharomyces cerevisiae DNATopoisomerase II. 1. A DNA-Dependent Burst in ATP Hydrolysis
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  • When bound to DNA, topoisomerase II from Saccharomycescerevisiae exhibits burst kineticswith respect to ATP hydrolysis. Pre-steady-state analysis showsthat the enzyme binds and hydrolyzestwo ATP per reaction cycle. Our data indicate that at least one ofthe two ATP is rapidly hydrolyzedprior to the rate-determining step in the reaction mechanism. WhenDNA is not bound to topoisomeraseII, the rate-determining step shifts to become either ATP binding orhydrolysis. Two possible mechanismsare proposed that agree with our observations.
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