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| - Transport and Deposition ofMetabolically Active and StationaryPhase Deinococcus radioduransin Unsaturated Porous Media
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| - Bioremediation is a cost-efficient cleanup technique thatinvolves the use of metabolically active bacteria to degraderecalcitrant pollutants. To further develop this techniqueit is important to understand the migration and depositionbehavior of metabolically active bacteria in unsaturatedsoils. Unsaturated transport experiments were thereforeperformed using Deinococcus radiodurans cells that wereharvested during the log phase and continuously suppliedwith nutrients during the experiments. Additional experimentswere conducted using this bacterium in the stationaryphase. Different water saturations were considered in thesestudies, namely 100 (only stationary phase), 80, and 40%.Results from this study clearly indicated that the physiologicalstate of the bacteria influenced its transport and depositionin sands. Metabolically active bacteria were morehydrophobic and exhibited greater deposition than bacteriain the stationary phase, especially at a water saturationof 40%. The breakthrough curves for active bacteria also hadlow concentration tailing as a result of cell growth ofretained bacteria that were released into the liquid phase.Collected breakthrough curves and deposition profileswere described using a model that simultaneously considersboth chemical attachment and physical straining. Newconcepts and hypotheses were formulated in this modelto include biological aspects associated with bacteria growthinside the porous media.
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