| Abstract
| - A sensitive high-performance ion chromatography (HPIC) method forseparation and quantitativedetermination of inositol mono- to hexaphosphate(IP1−IP6) isomers in selected foods andintestinalcontents is described. The method includes extraction of sampleswith HCl, separation of the inositolphosphates from the crude extract by anion-exchange chromatography,separation on high-performance ion-exchange columns with gradient elution in two combinedHPIC systems, anddetection using either postcolumn reaction and UV detection (system 1)or chemically suppressedconductivity detection (system 2).IP2−IP6 in samples were determined onthe first system, whichalso separated isomers of IP4 and IP5.Fractions of IP1−IP3 from the firstsystem were transferredto the second system after some additional sample preparation andanalyzed for isomers. Theapplicability and sensitivity of the method are illustrated bymeasuring the content of inositolphosphates in foods, ileal contents, and feces of humanbalances. Keywords: Inositol phosphate isomers; high-performance ion chromatography;HPIC; food;intestinal content
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