| Abstract
| - A method is described for the determination of the naturally derivedinsect control agent spinosadand its metabolites in soil, sediment, and water. The methoddetermines residues of the activeingredients in spinosad (spinosyns A and D) and two metabolites(spinosyn B and N-demethylspinosyn D). For soil and sediment, the method has a limit ofquantitation of 0.01 μg/g and a limit ofdetection of 0.003 μg/g. For water, the method has a limit ofquantitation of 0.001 μg/mL and alimit of detection of 0.0003 μg/mL. The analytes are extractedfrom water, soil, or sediment usingappropriate solvents, and the extracts are purified by liquid−liquidpartitioning and silica solidphase extraction. All four analytes are determined simultaneouslyin the purified extracts byreversed-phase high performance liquid chromatography with ultravioletdetection at 250 nm. Keywords: Spinosad; spinosyn A; spinosyn D; spinosad B; N-demethylspinosynD; soil; sediment;water; quantitation; HPLC-UV
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