| Abstract
| - Commercially prepared marigold flower (Tagetes erecta) extract was saponified and analyzed forcarotenoid composition. HPLC analyses were performed on two normal-phase columns (β-Cyclobondand silica) and on a C30 reversed-phase column. The extract contained 93% utilizable pigments(detected at 450 nm), consisting of all-trans and cis isomers of zeaxanthin (5%), all-trans and cisisomers of lutein, and lutein esters (88%). All were identified by chromatographic retention, UV−visible spectra, and positive ion electrospray mass spectrometry in comparison to authentic standards.Contrary to previous findings, insignificant levels (<0.3%) of lutein oxidation products were detectedin the saponified extract. This compositional determination is important for the application ofmarigold extract in nutritional supplements and increases its value as a poultry feed colorant becauseit contains more biologically useful lutein compounds than previously believed. Keywords: Lutein; marigold (Tagetes erecta); poultry; feed colorant
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