| Abstract
| - A C18 reversed-phase HPLC method has been developed to analyze the concentration of 2-O-β-d-glycopyranosyl-cucurbitacin E (synonym, elaterinide) in juice or reconstituted residues of juice derivedfrom a bitter mutant of Hawkesbury watermelon, Citrullus lanatus (Thunb.) Matsum. & Nakai.The method requires only two steps (homogenation and filtration) for sample preparation prior tocolumn injection and, thus, is amenable to processing large numbers of test samples. Percentelaterinide in juice samples from whole fruit, processed in various ways, ranged from 0.0230 to0.0808%. Error differences in percent elaterinide for repetitive analyses within the same lot of juiceranged from 1.2 to 5.6%. Keywords: HPLC analysis; corn rootworm; elaterinide; cucurbitacin E glycoside; Hawkesburywatermelon; Citrullus vulgaris Schrad.; Citrullus lanatus (Thunb.) Matsum. & Nakai.
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