| Abstract
| - A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. Theanalytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using differentcolumn chromatographic methods and used, as external standard, for the calibration of the methodand for quantification. The method was then applied to study the variation in SDG content inflaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG contentvaried between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g inwhole flaxseeds. Keywords: Secoisolarciresinol diglucoside; lignan; flaxseed; HPLC
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