| Abstract
| - An amarantin 11S globulin cDNA encoding one of the most important storage proteins of amaranthseeds, with a high content of essential amino acids, was expressed in Escherichia coli. A good levelof expression of recombinant amarantin with a molecular weight of 59 kDa was obtained. Therecombinant protein was extracted by ammonium sulfate precipitation and purified to homogeneityusing ion-exchange chromatography and reversed phase high-performance liquid chromatography.The expressed protein exhibited electrophoretic, immunochemical, and surface hydrophobicityproperties similar to those of native amarantin from amaranth seed. Also, the recombinant proteinwas refolded in vitro using two different methods. Keywords: Storage protein; amarantin; heterologous expression; recombinant amarantin; expressioninduction; immunodetection; in vitro refolding
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