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| - Immunoaffinity Sample Purification and MALDI-TOF MS Analysisof α-Solanine and α-Chaconine in Serum
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| - A sample purification technique was developed for the detection of potato glycoalkaloids (GAs)in blood serum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). GAs were extracted from spiked serum (5 mL) using a C18 solid-phase extractioncartridge. The GAs were then selectively captured on antibody-coated agarose beads. The agarosebeads were washed with water and the GAs eluted with 25 μL of methanol. MALDI-TOF MS wasused to detect the GAs in the methanol eluent. Immunoaffinity sample purification of the GAseffectively reduced the signal suppression observed during the analysis of unpurified samples.α-Chaconine and α-solanine were detected in serum spiked with 1 ng/mL of each GA. Keywords: Potato; glycoalkaloid; solanidine; antibody; affinity column; solid-phase extraction
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