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| - Adsorbed Protein Secondary and Tertiary Structures by CircularDichroism and Infrared Spectroscopy with Refractive IndexMatched Emulsions
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| - The secondary structure of protein adsorbed at the emulsion interface has been studied in refractiveindex matched emulsions using the techniques of circular dichroism (CD) and Fourier transforminfrared spectroscopy. Bovine serum albumin (BSA) and bovine β-lactoglobulin (βlg) stabilizedemulsions were studied, and the refractive index was altered by the addition of glycerol or polyethylene glycol. The effect of additive on the solution and adsorbed protein structure in addition tothe effect of adsorption time was considered. Both adsorption and glycerol addition alter proteinsecondary structure; however, the majority of secondary structure remains. Small changes are observed in the secondary structure of adsorbed protein with time. Near-ultraviolet CD studies showedthe effect of glycerol and adsorption on the aromatic groups. BSA showed small changes both uponthe addition of glycerol to protein in solution and upon adsorption. βlg showed slightly larger changesupon the addition of glycerol to protein in solution and a larger change upon adsorption. Keywords: Emulsion; secondary structure; β-lactoglobulin; BSA; CD; refractive index matchedemulsion
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