| Abstract
| - A set of haptens structurally resembling the herbicide imazethapyr (PURSUIT) was synthesized andused to derive monoclonal antibodies (MAbs) and direct and indirect competition enzyme immunoassays (EIAs) which could detect imazethapyr, imazaquin (SCEPTER), imazapic (CADRE), andimazamox (RAPTOR) in the 3−30 ng/mL (parts per billion) range, and imazapyr (ARSENAL) andimazamethabenz-methyl (ASSERT) in the 300−500 ppb range. Two MAbs, 3A2 and 3A5, had affinitiesof 10−75 nM for imazethapyr. MAbs 1A5, 1D2, and 3A5 were specific for the S isomers of theherbicides. Some MAbs were stable in solutions containing up to 15% methanol and 5% acetonitrilein indirect EIAs. Plates coated with hapten conjugates for indirect EIA could be stored frozen. Selectivityfor the imidazolinones by some MAbs varied with different coating conjugates. These MAbs andhaptens should prove useful in immunochemical analysis and residue recovery methods forimazethapyr and other imidazolinone herbicides. Keywords: Immunoassay; immunoaffinity; monoclonal antibody; imazethapyr; imidazolinone herbicide
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