| Abstract
| - Multiplex PCR reactions were developed for detecting simultaneously the CryIA(b) and pat genesfrom events 176, MON810, BT11, and T25 of transgenic maize, using only two pairs of primers, onefor the CryIA(b) gene and the other for the pat gene. The Roundup Ready soybean can be preciselydetected by a multiplex PCR reaction using known primers, amplifying fragments of the NOS andthe epsps sequences simultaneously. Transgenic events such as Roundup Ready soybean and GA21maize, among others, can be quantified by real-time PCR using a pair of primers and a probespecifically designed for annealing to the NOS ending region. As an alternative to amplifying anendogenous gene, the addition of a foreign gene in a percentage equal to the required level ofdetection, in a parallel reaction, is proposed. The use of hexane to homogenize large flour samplesis suggested. Keywords: GMOs; real-time PCR; maize; soybean; CryIA(b), PAT
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