A procedure is proposed for the supercritical fluid extraction of all-trans-lycopene from tomato usingcarbon dioxide at 40 °C without modifier. The present method minimizes the risk of degradation viaisomerization and oxidation of health-promoting ingredients, such as lycopene. The effect of differentexperimental variables on the solvating power of the supercritical fluid was evaluated in terms ofboth the selectivity achievable in the process and the yield of the extraction of all-trans-lycopene.Satisfactory separations of the all-trans-lycopene isomers from the cis counterparts were achievedusing a C30 column. The obtained extract contained 88% all-trans-lycopene and 12% cis-lycopene. Keywords: Supercritical fluid extraction; all-trans-lycopene; β-carotene; tomato