| Abstract
| - This study focused on the role of eggshell matrix proteins as a function of potential natural antimicrobialdefenses of avian eggs. The electrophoretic profile of SDS-PAGE showed that the soluble eggshellmatrix proteins had three major bands of 15 000, 36 000, and 66 000 and several minor bandscomprising 17 000, 25 000, 30 000, and 75 000, while insoluble matrix proteins were consisting ofvarious bands comprising at least 16 distinct migration bands between 10 000 and 200 000. Threebacteria species, Pseudomonas aureginosa, Bacillus cereus, and Staphylococcus aureus, were foundto be inhibited in the presence of soluble eggshell matrix proteins (100 μg/mL). On the other hand,Escherichia coli and Salmonella enteritidis were weakly inhibited at only an early stage of incubationtime (up to 4 h). Scanning electron microscopy revealed that eggshell matrix proteins might interactand disrupt the membrane integrity of bacteria. The present study clearly indicated that avian eggshellmatrix proteins possess a potential of novel antimicrobial defensin mechanism. Keywords: Avian egg; eggshell matrix proteins; antimicrobial defense; ovocleidin; lysozyme; gramnegative; gram positive; SEM
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