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| - ADP Ribosylation Factor Regulates Metabolism and AntioxidantCapacity of Transgenic Potato Tubers
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| - In our recent studies we have evidenced that repression of ADP-ribosylation factor (ARF) in potatoplants results in 14-3-3 gene activation. The significant alteration in plant phenotype and incarbohydrate content clearly indicates that there may also be changes in other metabolite syntheses.In this paper we present the data on contents of compounds, occurring in transgenic potato tubersfrom field trial, known to be important for the human diet. We also determine which of the ARF-antisense plant features resulted from ARF repression. This determination was accomplished by theanalysis of ARF-antisense plants transformed with cDNA encoding 14-3-3 protein in reverseorientation. The sucrose accumulation and the decrease in glycoalkaloids level were found to becharacteristic features of all transgenic plants. The increase in antioxidant capacity of transgenicpotato tubers should also be pointed out. The analysis of fat from modified potato tubers revealed anutritionally valuable composition of fatty acids, including the significant increase of linoleic acid level. Keywords: ARF; carbohydrates; 14−3−3 protein; transgenic plants; antioxidant potential
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