| Abstract
| - Validamycin A, a main component of the antibiotic validamycin complex, which is widely used tocontrol sheath blight disease of rice plants, can be determined by capillary zone electrophoresis withindirect UV detection. The influence of various separation conditions including background electrolyteand modifier concentration, pH, and voltage was investigated. By using 10 mM aminopyrine−2 mMethylenediaminetetraacetic acid at pH 5.2 as the carrier electrolyte, high efficiency separation ofvalidamycin A was achieved with the number of theoretical plates up to 350 000 plates/m. The linearrange was across 3 orders of magnitude. The relative standard deviations for migration times andpeak areas were less than 0.5 and 3.0%, respectively. The limit of detection for validamycin A was1.0 μg/mL. The average recoveries ranged from 103.0 to 104.8%. This method has many advantagesas compared with high-performance liquid chromatography and micellar electrokinetic capillarychromatography in the determination of commercial formulations. Keywords: Validamycin A; capillary zone electrophoresis (CZE); indirect UV detection
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