| Abstract
| - The objectives of this study were to optimize a monoclonal competitive indirect enzyme-linkedimmunosorbent assay (CI-ELISA) for hexanal detection, optimize solubilization and alkylationprocedures for the formation of hexanal-protein adducts, and compare the ability the CI-ELISA,thiobarbituric acid reactive substances assay (TBARS), and a solid-phase microextraction−gaschromatography−mass spectrometry (GC/MS-SPME) method for monitoring lipid oxidation in freeze-dried chicken protein. Freeze-dried myofibrils with added methyl linoleate (0.6 mmol/g of protein)were stored at 50 °C at two water activities (aw = 0.30 and 0.75) for 5 days. Hexanal was measuredby GC/MS-SPME and CI-ELISA, and malonaldehyde by TBARS. At an aw of 0.30, 34.7 and 39.7 μgof hexanal/g of myofibril were detected by GC/MS-SPME and CI-ELISA, respectively, after 4 days ofstorage. At an aw of 0.75, 39.8 and 61.1 μg of hexanal/g of myofibril were detected by GC/MS-SPMEand CI-ELISA, respectively, after 4 days of storage. The CI-ELISA was well correlated with the GC/MS-SPME (r = 0.78) and TBARS (r = 0.87) methods. The correlation of the hexanal-specific CI-ELISA to both GC/MS-SPME and TBARS verified the ability of the CI-ELISA to be used as an indexof lipid oxidation, offering the convenience for use in a kit to be utilized within a food-processingfacility. Keywords: Lipid oxidation; hexanal; immunoassay; chicken
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