| Abstract
| - Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type I) with an IC50 of 1.3 μM. Theinhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity.The inhibition kinetics analyzed by Lineweaver−Burk plots indicates that octyl gallate is a competitiveinhibitor, and the inhibition constant, KI, was obtained as 0.54 μM. One molecule of octyl gallatescavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation.In addition, octyl gallate was effective in preventing lipid peroxidation. Keywords: Soybean lipoxygenase; octyl gallate; competitive inhibitor; Lineweaver−Burk plots;antioxidant
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