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| - Determination of Phytic Acid and Inositol Pentakisphosphatesin Foods by High-Performance Ion Chromatography
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| - A high-performance anion exchange chromatographic method was adapted for the quantitativedetermination of phytic acid and inositol pentakisphosphate isomers (excluding enantiomers) in foods.Because of the cost and limited availability of inositol phosphate standards, a phytic acid sodium saltstandard was used for the calculation of an average relative response factor for the quantification ofinositol pentakisphosphate isomers, and the purity of phytic acid sodium salt standard was alsoaccurately established. The detection limits (S/N = 3) for phytic acid and inositol pentakisphosphateswere in the range of 1.5−3.4 μM (0.1−0.2 μg/100 μL). This method has been successfully appliedto the determination of phytic acid and inositol pentakisphosphates in a variety of beans and nutsafter extraction with 0.5 M HCl and cleanup with solid phase extraction cartridges. The resultsdemonstrated that there was a strong correlation between either the phytic acid content or the totalcontent of phytic acid together with inositol pentakisphosphates and the total dietary fiber content inthe group of all raw dry beans and in the group of raw dry black beans but not in the group of rawdry red kidney beans, which was probably due to the insufficient number of the raw dry red kidneybean samples. Keywords: Phytic acid; inositol pentakisphosphate; high-performance ion chromatography; food; bean;nut
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