| Abstract
| - Bisphenol A [2,2-bis(4-hydroxyphenyl)propane] (BPA) is a widely used industrial chemical resultingin occupational and consumer exposure. BPA possesses weak estrogenomimetic activity and canbe cytotoxic, though the underlying mechanisms of its toxicity toward cells are not completelyunderstood. The metabolism of BPA by CD1 mice liver microsomal and S9 fractions was investigated.Nine metabolites were isolated and characterized using HPLC and mass spectrometry. Many of thesemetabolites were characterized for the first time in mammals, namely isopropyl-hydroxyphenol(produced by the cleavage of BPA), a bisphenol A glutathione conjugate, glutathionyl-phenol,glutathionyl 4-isopropylphenol, and BPA dimers. Most of these metabolites apparently share a commonmetabolic pathway, for which considerable evidence supports the hypothesis of the production of areactive intermediate, and also helps explain BPA cytotoxicity. Keywords: BPA; in vitro metabolism; mouse; endocrine disruptor
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