Abstract
| - Antioxidant properties of marjoram (Origanum majorana L.) herb and extracts obtained with ethanol,n-hexane, and supercritical CO2 extraction are presented. Individual antioxidants, ursolic acid, carnosicacid, and carnosol, were quantified with high-performance liquid chromatography. The effects ofdifferent parameters (temperature and pressure) of high-pressure extraction on the yield of carnosolwere studied. Furthermore, two marjoram herbs from Hungary and Egypt were compared measuringhydrogen-donating abilities with 1,1-diphenyl-2-picrylhydrazyl by spectrophotometric and the totalscavenger capacities by chemiluminometric methods from the aqueous extracts of the herbs. Theantioxidant activities of the solvent extracts were performed using the Rancimat method. The Egyptianherb and its extracts possessed better antioxidant activities than Hungarian ones. Applying supercriticalCO2 extraction, the highest value of carnosol was obtained at 400 bar and 60 °C. Keywords: Marjoram (Origanum majorana L.); antioxidant properties; DPPH method; H2O2/OH•-microperoxidase-luminol system; Rancimat method; ursolic acid; carnosol and carnosic acid
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