| Abstract
| - Ribosomal intergenic spacer analysis (RISA) has been applied to the microbial community analysisof agronomic products in combination with a simple and rapid DNA extraction method, consisting ofa one-step extraction and two-step purification, for a variety of agronomic products. RISA appears tobe a useful tool for the study of the community structures of food-associated microbes and their useas a unique fingerprinting signature for each agronomic product. Sequencing analyses of ampliconsgenerated from RISA suggest that this method can detect conventional microbes. In the case ofRISA of wasabi paste DNA, the sequences of the amplicons showed high similarity to the plantpathogen Xanthomonas campestris and the soil bacterium Bacillus subtilis, whereas several food-associated bacteria (Lactococcus lactis, Lactococcus raffinolactis, and Lactococcus sakei) weredetected using this technique in sausage DNA. Unexpectedly, the sequencing analyses also revealedthe presence of several microbes that possessed high similarity to human bacterial pathogens suchas Weissella confusa and Yersinia pestis. The results suggest that RISA will be a useful method forroutine microbial community analysis in agronomic products. Keywords: Microbial community analysis; ribosomal intergenic spacer analysis
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