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| - Linolenic Acid-Modified Chitosan for Formation ofSelf-Assembled Nanoparticles
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| - Chitosan was modified by coupling with linolenic acid through the 1-ethyl-3-(3-dimethylaminopropyyl)carbodiimide-mediated reaction. The degree of substitution was measured by 1H NMR, and it was1.8%, i.e., 1.8 linolenic acids group per 100 anhydroglucose units. The critical aggregationconcentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined bymeasuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value inphosphate-buffered saline (PBS) solution (pH 7.4) was 5 × 10-2 mg/mL. The average particle sizeof self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm witha unimodal size distribution ranging from 100 to 500 nm. A transmission electron microscopy studyshowed that the formation of near spherical shape nanoparticles had enough structural integrity.The loading ability of hydrophobically modified chitosan (LA-chitosan) was investigated by using bovineserum albumin (BSA) as a model protein. Self-aggregated nanoparticles exhibited an increased loadingcapacity (19.85 ± 0.04 to 37.57 ± 0.25%) with an increasing concentration of BSA (0.1−0.5 mg/mL). Keywords: Chitosan; linolenic acid; nanoparticles; self-aggregates; BSA
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