| Abstract
| - The water specimens were collected from wells and irrigation ditches in the agricultural area to thesouth of Milan and from Olona River and Mantua Lake and analyzed for paraquat detection. Theassay was performed using a specific polyclonal antibody raised in sheep and rabbit anti-sheep IgGconjugated with a chelating molecule 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylicacid complexed with Eu3+ as a fluorescent marker. Bovine serum albumin conjugated with 5-(1‘-methyl[4,4‘]bipyridinyl-1-yl)pentanoic acid was used in solid phase preparation. The sensitivity achievedwas 20 ng L-1. The recovery in samples spiked with three different PQ concentrations was between88 and 108%. Keywords: Paraquat; bipyridylium herbicides; immunoassay; europium chelate; time-resolved fluorescence
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