| Abstract
| - The inhibitory activity of Curcuma longa L. (turmeric) rhizome constituents against sortase A, a bacterialsurface protein anchoring transpeptidase, from Staphylococcus aureus ATCC 6538p was evaluated.The activity of the isolated compounds (1−4) was compared to that of the positive control,p-hydroxymecuribenzoic acid (pHMB). The biologically active components of C. longa rhizome werecharacterized by spectroscopic analysis as the curcuminoids curcumin (1), demethoxycurcumin (2),and bisdemethoxycurcumin (3). Curcumin was a potent inhibitor of sortase A, with an IC50 value of13.8 ± 0.7 μg/mL. Bisdemethoxycurcumin (IC50 = 31.9 ± 1.2 μg/mL) and demethoxycurcumin (IC50= 23.8 ± 0.6 μg/mL) were more effective than pHMB (IC50 = 40.6 ± 1.2 μg/mL). The three isolatedcompounds (1-3) showed no growth inhibitory activity against S. aureus strain Newman, with minimuminhibitory concentrations (MICs) greater than 200 μg/mL. Curcumin also exhibited potent inhibitoryactivity against S. aureus cell adhesion to fibronectin. The suppression of fibronectin-binding activityby curcumin highlights its potential for the treatment of S. aureus infections via inhibition of sortaseactivity. These results indicate that curcumin is a possible candidate in the development of a bacterialsortase A inhibitor. Keywords: Curcuma longa L.; curcumin; Staphylococcus aureus; sortase inhibitory activity; sortaseA; antibacterial activity; fibronectin-binding activity; curcuminoids
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