| Abstract
| - Isothiocyanates (ITCs) found in cruciferous vegetables are potentially important anticarcinogenicphytochemicals for many types of cancers including breast cancer. In this study, we have shownthat three isothiocyanates, sulforaphane, erucin, and iberin, are potent inducers of thioredoxinreductase 1 (TrxR1) in human breast cancer MCF-7 cells. Sulforaphane, erucin, and iberin at 1 μMinduce TrxR1 mRNA 2−3-fold within 8 h of treatment, and induce mRNA 5−7-fold with 12 μM ITCtreatments. Selenium did not affect sulforaphane-induced TrxR1 mRNA levels, but significantlyenhanced both TrxR1 protein expression (up to 9-fold in erucin treatment) and corresponding activities.These results suggest that dietary ITCs are important factors in the regulation of redox status throughthe induction of the selenoprotein thioredoxin reductase. Keywords: Isothiocyanates; sulforaphane; erucin; iberin; selenium; thioredoxin reductase
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