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| - Nuclear Magnetic Resonance Spectroscopic Based Studies ofthe Metabolism of Black Tea Polyphenols in Humans
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| - Epidemiological studies indicate that a high intake of flavonoids is associated with an improved healthstatus. Tea is one of the most abundant sources of flavonoids in the human diet. The bioavailabilityand biotransformation of tea flavonoids are, however, not clearly understood. The aim of the presentstudy was to investigate the metabolism of black tea via a nonspecific screening method. 1H nuclearmagnetic resonance (NMR) spectroscopy was used to obtain nonselective profiles of urine samplescollected from three human volunteers before and after a single dose of black tea. The complexspectroscopic profiles were interpreted with the use of pattern recognition techniques. Hippuric acidwas confirmed as the major urinary black tea metabolite. One previously unknown metabolite wasdetected and identified as 1,3-dihydroxyphenyl-2-O-sulfate (sulfate conjugate of pyrogallol) using HPLCdirectly coupled to mass spectrometry and 1H NMR spectroscopy. This study shows that NMR-patternrecognition studies can be used for the discovery of unknown flavonoid metabolites in humans. Keywords: Metabonomics; NMR spectroscopy; urine; black tea; polyphenols; phenolic acids; principalcomponents analysis; metabolism; bioavailability
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