| Abstract
| - Ovalbumin gene Y has been known as a member of the ovalbumin gene family since 1982, when itsencoding gene was sequenced. In the present study, ovalbumin gene Y has been demonstrated asa new minor protein of hen egg white. This protein has been isolated by isoelectrofocalization andtwo-dimensional polyacrylamide gel electrophoresis and has been characterized using peptide massfingerprinting. The concentration ratio of ovalbumin gene Y:ovalbumin is about 13:100. Unlikeovalbumin, ovalbumin gene Y is not phosphorylated, but like ovalbumin, this protein is glycosylated.Ovalbumin gene Y exists as a mixture of three molecular species, which differ in their isoelectricpoints. The polymorphism of this protein cannot be explained by various glycosylation levels. Keywords: Hen egg white; ovalbumin gene Y; isoelectrofocalization; two-dimensional electrophoresis;mass spectrometry
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