| Abstract
| - Structured lipids resembling human milk fat (HMF) enriched with omega-3 fatty acids were synthesizedby enzymatic acidolysis reactions between tripalmitin, hazelnut oil fatty acids (FA), and omega-3 FAconcentrate. Response surface methodology was used to model and optimize the incorporation ofomega-3 FA and oleic acid into tripalmitin, in hexane, using immobilized sn-1,3-specific lipase,Lipozyme RM IM. The three factors chosen were substrate molar ratio, reaction temperature, andreaction time. Good quadratic models were obtained for the incorporation of eicosapentaenoic acid(EPA) plus docosahexaenoic acid (DHA) (response 1) and oleic acid (response 2) by multipleregression and backward elimination. The determination coefficient (R2) value for the models was0.95. The adjusted R2 values were 0.91 and 0.92 for responses 1 and 2, respectively. The optimalconditions generated from the models for the targeted total EPA and DHA (5%) and oleic acid (40%)incorporation were 12.4 mol/mol, 55 °C, and 24 h for substrate ratio, temperature, and time,respectively. The model was verified, which led to the production of a HMF ingredient with 76.6%palmitic acid at the sn-2 position. Keywords: Hazelnut oil; human milk fat substitutes; lipase-catalyzed acidolysis; Lipozyme RM IM;omega-3 fatty acids; response surface methodology
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