| Abstract
| - 5-(Hydroxymethyl)-2-furfural (I) is a major breakdown product occurring in solutions with highconcentrations of fructose and glucose and is present in many fruit juices, in heat-sterilized parenteralsolutions, and in baby cereals. The objective of this study was to characterize and identify5-(hydroxymethyl)-2-furfural metabolites in human subjects following the consumption of dried plumjuice and/or dried plums. Subjects were fasted overnight and blood and urine samples were obtainedduring the day following consumption. Subjects fed the dried plum juice and dried plums consumed3944 μmol (497 mg) and 531 μmol (67 mg) of I, respectively. Four presumed metabolites of I weredetected in the urine of subjects that consumed dried plum juice. They were tentatively identifiedusing HPLC-MS/MS as (1) N-(5-hydroxymethyl-2-furoyl)glycine (III), (2) 5-hydroxymethyl-2-furoic acid(II), (3) (5-carboxylic acid-2-furoyl)glycine (IV), and (4) (5-carboxylic acid-2-furoyl)aminomethane (V).Total urinary excretion during the 6 h following the consumption of dried plum juice was 168, 1465,137, and 75 μmoles on the basis of II as a standard for II, III,IV, and V, respectively. The estimatedtotal recovery of I metabolites was 46.2% and 14.2% of the I dose during the first 6 h after consumptionof dried plum juice and dried plums, respectively. I seems to be metabolized rapidly to glycineconjugates and other metabolites and excreted in the urine. Keywords: Hydroxymethylfurfural; prunes; human subjects
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