| Abstract
| - A high-throughput relative 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging capacity (RDSC)assay was developed and validated in the present study. This RDSC assay is easy to perform andhas acceptable accuracy (90−110% recovery), precision [3.9−7.0% pooled relative standard deviation(RSD)], and reproducibility (2.2 and 3.5% interday and intraday RSD). This assay reports the RDSCvalues for antioxidant samples, which make it possible to compare the DPPH radical scavengingcapacities of antioxidants determined in different laboratories. The RDSC assay may be conductedin aqueous alcohol and acetone for hydrophilic antioxidants or in the organic solvents for lipophilicantioxidants without solubilizing agents, which makes it possible to directly compare the radicalscavenging capacities of hydrophilic and lipophilic antioxidants. In addition, the high-throughput RDSCassay could be utilized for EC50 value estimation. The high-throughput RDSC assay may be used forscreening and investigating potential natural antioxidants. Keywords: Radical scavenging capacity; RDSC, DPPH radical; antioxidant activity; high-throughput;AUC
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