| Abstract
| - Diallyl disulfide (DADS) is an organosulfur compound from garlic, which inhibits colon tumor cellproliferation. In a previous study, we have shown that in Caco-2 and HT-29 cells DADS (200 μM)increases global histone acetylation, CDKN1A mRNA, and p21waf1 protein levels and induces G2/Mcell cycle arrest. These results suggested that DADS could inhibit cell proliferation through at leastin part a transcriptional activation of CDKN1A expression involving histone acetylation. In this study,using chromatin immunoprecipitation assays, we demonstrate that in Caco-2 and HT-29 cells histoneH4 and/or H3 acetylation is increased within CDKN1A promoter after 3 and 6 h treatments with DADS.These results strongly suggest that histone acetylation, a molecular mechanism implicated in theregulation of gene expression, could account for the induction of CDKN1A expression and theantiproliferating effects of DADS in colon tumor cells. Keywords: Diallyl disulfide; histone acetylation; human colon tumor cells; chromatin immunoprecipitation; CDKN1A gene
|
