| Attributs | Valeurs |
|---|
| type
| |
| Is Part Of
| |
| Subject
| |
| Title
| - Determination of Tetracycline Antibiotic Residues in EdibleSwine Tissues by Liquid Chromatography withSpectrofluorometric Detection and Confirmation by MassSpectrometry
|
| has manifestation of work
| |
| related by
| |
| Author
| |
| Abstract
| - A sensitive and specific method is described for the simultaneous determination of oxytetracycline,tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquidchromatography with spectrofluorometric and mass spectrometry detection. The procedure involveda preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phaseextraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performedwith spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits ofquantification were 50 μg/kg for muscle and 100 μg/kg for kidney tissues. The recovery values weregreater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used forthe quantification of tetracyclines in swine tissues samples. The selective liquid chromatography massspectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample wasmade by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gavethe protonated molecular ion and two typical fragment ions, required for their confirmation in singleion monitoring scan mode in animal tissues. Keywords: Tetracyclines; swine tissues; liquid chromatography; spectrofluorometric detection; massspectrometric detection
|
| article type
| |
| is part of this journal
| |
