| Abstract
| - Since angiogenesis is involved in initiating and promoting several diseases such as cancer andcardiovascular events, this study was designed to evaluate the anti-angiogenesis of low-molecular-weight (LMW), highly sulfated λ-carrageenan oligosaccharides (λ-CO) obtained by carrageenandepolymerization, by CAM (chick chorioallantoic membrane) model and human umbilical veinendothelial cells (HUVECs). Significant inhibition of vessel growth was observed at 200 μg/pellet. Ahistochemistry assay also revealed a decrease of capillary plexus and connective tissue in λ-COtreated samples. λ-CO inhibited the viability of cells at the high concentration of 1 mg/mL, whereasit affected the cell survival slightly (>95%) at a low concentration (<250 μg/mL), and HUVEC is themost sensitive to λ-CO among three kinds of cells. Furthermore, the inhibitory action of λ-CO wasalso observed in the endothelial cell invasion and migration at relatively low concentration (150−300μg/mL), through down-regulation of intracellular matrix metalloproteinases (MMP-2) expression onendothelial cells. Taken together, these findings demonstrate that λ-CO is a potential angiogenesisinhibitor with combined effects of inhibiting invasion, migration, and proliferation. Keywords: Angiogenesis; λ-carrageenan; oligosaccharides; chick chorioallantoic membrane; humanumbilical vein endothelial cells
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