| Abstract
| - A polyclonal anti-gatifloxacin antibody has been prepared, and an indirect competitive enzyme-linkedimmunosorbent assay (cELISA) was developed on the basis of the antibody prepared for the firsttime. The antibody shows high sensitivity with an IC50 value of 2.6 ppb and excellent specificity withonly a minor cross-reaction with lomefloxacin (3.0%) among common (fluoro)quinolones evaluatedin this study. The high specificity of the antibody was explained by the molecular structures of relateddrugs by comparison with published research. The cELISA test kit developed has a detection limit of0.05 ppb and could be used as a screening method to detect and regulate illegal use of gatifloxacinin food and food products. The test kit was applied to the detection of milk samples spiked bygatifloxacin. The recovery rates were in the range of 86−106%, whereas the intra- and interassaycoefficients of variation were <14.3 and <19.6%, respectively. Keywords: Gatifloxacin; drug residue; antibody; immunoassay; cross-reactivity
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