| Abstract
| - Polysaccharides isolated from Phellinus linteus were chemically modified by carboxymethylation, andthe structural and physiological properties of the derivative were investigated. 13C NMR spectroscopyshowed that the polysaccharides extracted from P. linteus contained (1−3)-β-glucans with a (1−6)-linkage. The carboxymetehylation of the P. linteus polysaccharides was confirmed by Fourier transforminfrared spectroscopy, and the degree of substitution was obtained by the potentiometric titration,which was calculated to be 0.63. The bronchoalveolar lavage experiments showed that thecarboxymethylated derivative raised the nitric oxide production. In addition, the carboxymethylationstimulated in vitro cytotoxic activity against the HT1080 cell line. Thus, the derivative exhibited theenhanced activity of immune systems, which would be explained by the improved water solubilityand structural changes by carboxymethylation. However, a slight decrease in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of the derivative was observed. Keywords: Phellinus linteus; carboxymethylation; cytotoxic activity; nitric oxide; DPPH radicalscavenging activity
|
