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| Title
| - Inhibition of Lactoperoxidase-Catalyzed 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and Tyrosine Oxidation by Tyrosine-Containing Random Amino Acid Copolymers
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| Abstract
| - Oxidation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) by lactoperoxidase was found to be inhibited by tyrosine-containing random amino acid copolymers but not by tyrosine. Both electrostatic effects and polymer size were found to be important by comparison of negatively and positively charged copolymers of varying lengths, with poly(Glu, Tyr)4:1 ([E4Y1]∼40) as the strongest competitive inhibitor (EC50 ∼20 nM). This polymer did not form dityrosine in the presence of lactoperoxidase (LPO) and peroxide. Furthermore, incubation with tert-butyl hydroperoxide, as opposed to hydrogen peroxide, resulted in a peculiar long lag phase of the reaction between the redox intermediate compound II and [E4Y1]∼40, indicating a very tight association between enzyme and inhibitor. We propose that interactions between multiple positively charged areas on the surface of LPO and the polymer are required for optimal inhibition.
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| Alternative Title
| - Inhibition of LPO by Tyrosine-Containing Polymers
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