| Abstract
| - The cytotoxicity and photocytotoxicity of trans-[RuCl2(DMSO)4] and cis-[RuCl2(DMSO)4]complexes was tested in two melanoma cell lines, human (SK-MEL 188) and mouse (S91). Thetrans isomer was found to be more effective for cell growth inhibition than its cis analogueboth in the presence and in the absence of illumination. However, the antiproliferative activityof both isomers was significantly enhanced after irradiation with UVA light in comparisonwith their activity observed in the dark. The influence of light on the reaction of bothruthenium(II) isomers with the single-stranded hexanucleotide d(T2GGT2), chosen as a modelsystem for DNA, was also studied using chromatography and mass spectrometry techniques.The photochemical reaction of the ruthenium(II) complexes with the oligonucleotide d(T2GGT2)resulted in the formation of Ru(G−N7)2 adducts, which was not observed in the same timescale in thermal reactions. The initial short irradiation of the inert cis isomer was found tofacilitate the covalent adduct formation with d(T2GGT2) in the secondary thermal reactionsand with a rate comparable to that found for the trans isomer, which is ca. 5−10 times morereactive in the dark.
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