| Abstract
| - Impedance spectroscopy is used to characterize the structure of biomaterial layers on the gate surface ofISFET devices, and to elucidate antigen−antibody binding interactions on the gate interface. The method isbased on the recording of the transfer function of the system as a function of frequency and on the extractionof the respective τ1 and τ2 values of the systems. From the experimental values, the film thicknesses of therespective protein layers are estimated. The method is applied to elucidate the structures of a glucose oxidase,GOx, multilayer assembly, and of a di-biotin-cross-linked avidin multilayer system. The method is also usedto sense the dinitrophenyl antibody by following the formation of the antigen−antibody complex on the gatesurface.
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