| Abstract
| - Tris(2,2‘-bipyridyl)ruthenium(II) (Ru(bpy)32+) is generally considered as the most valuable electrochemiluminescence agent. For better utilization of this agent, various immobilization methods in different matrixeshave been developed. However, its application is still subject to low stability and low sensitivity. Herein, wereport on the fabrication of a three dimensionally (3D) ordered macroporous gold film electrode as the matrixfor efficient immobilization of Ru(bpy)32+ in zirconia-Nafion sol−gel film. The 3D macroporous gold filmwith good conductivity has a large active surface area and interconnected macroporous structure, which enhanceloading of the electrochemiluminescence (ECL) agent and mass transport of the analytes. In addition, Ru(bpy)32+ immobilized on the macroporous gold electrodes is more electrochemically accessible than it is onflat gold electrodes. These effects enable us to obtain high sensitivity of the presented sensor. As ademonstration, tripropylamine (TPA) was used as the model compound. Excellent performance of the presentedsensor can be achieved, for example, the ECL response of the sensor to TPA shows a wide linear range from1.0 nM to 10.0 μM with a detection limit down to 0.5 nM.
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