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À propos de : First Preparation of Biotinylated Gradient PolyethyleneSurface to Bind Photoactive Caged Streptavidin        

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  • First Preparation of Biotinylated Gradient PolyethyleneSurface to Bind Photoactive Caged Streptavidin
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  • A gradient polyethylene (PE) surface was created through corona treatment, in which the corona powerincreased along the 5 cm length of the PE. The gradient surface was treated with polyethyleneimine andthen biotin. Fluorescein-conjugated streptavidin (SAV) caged within 5-carboxymethoxy-2-nitrobenzyl(CMNB) adsorbed onto the biotinylated gradient PE surface following molecular recognition principles.Photoirradiation decomposed the CMNB cage and allowed the fluorescein-conjugated SAV to fluoresce,the intensity of which increased gradually along the PE surface.
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