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| - Displacement of Molecules near a Metal Surface as Seen byan SPR−SPFS Biosensor
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| - Movement of a fluorophore-labeled antibody on the surface of a self-assembled monolayer (SAM) wasobserved by surface plasmon resonance and surface-plasmon field-enhanced fluorescence spectroscopy(SPFS). At an extremely low surface coverage, the antibody lies close to the biotin-functionalized SAMsurface. As additional nonlabeled antibodies were bound, the fluorophore-labeled antibody was displacedaway from the SAM surface (and thus the gold surface) due to the constraint imposed by the neighboringnonlabeled antibody. A greater SPFS fluorescence signal was observed due to the weaker fluorescencequenching at large distances from the gold surface. The magnitude of the displacement is proportionalto the available biotin binding sites on the sensor surface. The displacement is theoretically explained onthe basis of the relationship between the fluorescence intensity and the evanescent field amplitude withinthe dielectric medium.
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