We report in this paper an electric lithographic (EL) technique to generate protein patterns with sub-micrometerresolution on a poly(N-tBOC-2-aminoethyl methacrylate) surface. In the EL process, an electric potential is appliedbetween metal patterns on a mask and the poly(N-tBOC-2-aminoethyl methacrylate) layer to electrochemically inducethe dissociation of the tBOC from the amine functional groups. Proteins are then selectively attached to the aminefunctional groups in the modified polymer surface areas to form protein patterns. This technique can reliably generatehigh-resolution protein patterns down to ∼300 nm on the polymer surface at a high speed with a simple process/system.