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| - β-endorphin inhibits the production of interleukin-8 by human chorio-decidual cells in culture
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| - Interleukin-8 (IL-8) is produced by human decidual cells in culture, and may play a role in the initiation of parturition. β-endorphin is released in significant amounts into the maternal and fetal circulation during labour. The effect of β-endorphin on IL-8 production by human chorio-decidual cells in culture was investigated. Mixed cells were obtained from the decidual surfaces of 35 term placentas. The cells were plated out at 10×106 cells per well in Roswell Park Memorial Institute 1640 culture medium. After 48 h the cells were washed and incubated with either plain culture medium (control), 1 μmol/l progesterone, 1-100 nmol/l β-endorphin or 1 nmol/l N-acetyl β-endorphin. After 48 h, IL-8 concentrations were measured in the supernatants by enzyme-linked immunosorbent assay (ELISA). Experiments were repeated in the presence of naloxone (1 μmol/l) and using calcium-deficient culture medium. Progesterone (P< 0.0002) and β-endorphin (P< 0.0005) significantly inhibited the production of IL-8. The inhibitory effect of β-endorphin was blocked by naloxone and by using calcium-deficient medium. N-acetyl β-endorphin had no significant effect on IL-8 production. These findings suggest that β-endorphin has an inhibitory effect on IL-8 production by decidual cells, and that the effect is mediated via opioid receptors and is calcium-dependent.
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