| Abstract
| - Rumelenic (cis-9,trans-11,cis-15 18:3) acid is a naturally occurring conjugated isomer of α-linolenicacid (CLnA) in milk fat. Metabolism in rats was studied using a synthetic CLnA mixture, composedmainly by equimolar quantities of cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15 CLnA isomers. Theirmetabolisms were studied by feeding high quantities of CLnA (150 mg/day) for 4 days to rats thathad been reared on a fatfree diet for 2 weeks. After this period, animals were sacrificed and liver andepididymal adipose tissue lipids extracted. Six metabolites of the cis-9,trans-11,cis-15 18:3 CLnAisomers were identified as being cis-7,trans-9,cis-13 16:3, cis-11,trans-13,cis-17 20:3, cis-8,cis-11,trans-13,cis-17 20:4, cis-5,cis-8,cis-11,trans-13,cis-17 20:5, cis-7,cis-10,cis-13,trans-15,cis-19 22:5, and cis-4,cis-7,cis-10,cis-13,trans-15,cis-19 22:6 acids. Two metabolites of cis-9,trans-13,cis-1518:3 CLnA isomer were also identified by GC-MS as being cis-7,trans-11,cis-13 16:3 and cis-5,cis-8,cis-11,trans-15,cis-17 20:5. Keywords: Conjugated linolenic acid; fatty acids; lipid metabolism; mass spectrometry
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