| Abstract
| - The effect of a commercial Phaseolus vulgaris extract (PVE, starch stopper) on ileal and fecalendogenous protein losses was studied. Growing rats were fed for 14 days a protein-free dietcontaining PVE at a nutritional concentration of 0% (PF1), 0.4% (PF2), or 1.1% PVE (PF3) or 1.1%autoclaved PVE (PF4). An indigestible marker (TiO2) was included in each diet. Ileal endogenousamino acid (AA) losses were significantly higher (P< 0.05) in PF3 (20% higher than in PF1), exceptfor Pro, Gly, Ala, and His. Endogenous ileal N losses were 22% higher in PF3 than in PF1. Endogenousfecal AA and N losses were all significantly higher (P< 0.05) in PF3. Starch digestibility (∼100%),food intake (single daily meal, d10−23), and body weight loss were not significantly different amongthe groups. PVE, at 1.1% of the diet, not only was ineffective in reducing starch digestibility but alsoled to increased ileal endogenous N losses, possibly due to the antinutritional factors (trypsin inhibitor,lectin) present in the PVE. Keywords: Gut endogenous losses; amino acid; nitrogen; Phaseolus vulgaris; rat; antinutritional factors
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